HORDB

General Information

ID: hor005763
Uniprot ID: P01042
Protein name: Low molecular weight growth-promoting factor
Gene name: KNG1
Organism: Homo sapiens (Human)
Family: NA
Source: Human
Expression: Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
Disease: Diseases associated with KNG1 include High Molecular Weight Kininogen Deficiency and Angioedema, Hereditary, 6.
Comments: NA
Taxonomy: Homo (genus), Homininae (subfamily), Hominidae (family), Hominoidea (superfamily), Catarrhini (parvorder), Simiiformes (infraorder), Haplorrhini (suborder), Primates (order), Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
GO MF: GO:0004869 cysteine-type endopeptidase inhibitor activity; GO:0005102 signaling receptor binding; GO:0005179 hormone activity; GO:0005515 protein binding; GO:0008201 heparin binding; GO:0008270 zinc ion binding; GO:0030414 peptidase inhibitor activity
GO BP: GO:0006954 inflammatory response; GO:0007162 negative regulation of cell adhesion; GO:0007165 signal transduction; GO:0007204 positive regulation of cytosolic calcium ion concentration; GO:0007596 blood coagulation; GO:0007599 hemostasis; GO:0030195 negative regulation of blood coagulation; GO:0042311 vasodilation
GO CC: NA

Sequence Information

Sequence: WGHE
Length: 4
Propeptide: MKLITILFLCSRLLLSLTQESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTVSPPHTSMAPAQDEERDSGKEQGHTRRHDWGHEKQRKHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKKNGKHNGWKTEHLASSSEDSTTPSAQTQEKTEGPTPIPSLAKPGVTVTFSDFQDSDLIATMMPPISPAPIQSDDDWIPDIQIDPNGLSFNPISDFPDTTSPKCPGRPWKSVSEINPTTQMKESYYFDLTDGLS
Signal peptide: MKLITILFLCSRLLLSLT
Modification: NA
Glycosylation: NA
Mutagenesis: NA

Activity

Function: Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.; [Bradykinin]: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
Mechanism: NA
Cross BBB: NA
Target: BDKRB2
Target Unid: P30411
IC50: NA
EC50: NA
ED50: NA
kd: NA
Half life: NA

Structure

Disulfide bond: NA
Structure ID: AF-P01042-F1(AlphaFold_DB_ID)

Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)

hor005763_AF2.pdb

hor005763_ESM.pdb

Physical Information

Mass:	58123	Formula:	C24H29N7O7
Absent amino acids:	ACDFIKLMNPQRSTVY	Common amino acids:	EGHW
pI:	5.36	Basic residues:	1
Polar residues:	1	Hydrophobic residues:	1
Hydrophobicity:	-200	Boman Index:	-820
Half-Life:	2.8 hour	Half-Life Yeast:	3 min
Half-Life E.Coli:	2 min	Aliphatic Index	0
Instability Index:	-1842.5	Extinction Coefficient cystines:	5500
Absorbance 280nm:	1833.33

Literature

PubMed ID: 6441591
Title: Isolation of a human cDNA for alpha 2-thiol proteinase inhibitor and its identity with low molecular weight kininogen.
PubMed ID: 2989293
Title: Cloning and sequence analysis of cDNAs for human high molecular weight and low molecular weight prekininogens. Primary structures of two human prekininogens.
PubMed ID: 14702039
Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.
PubMed ID: 16641997
Title: The DNA sequence, annotation and analysis of human chromosome 3.
PubMed ID: 15489334
Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
PubMed ID: 3484703
Title: Completion of the primary structure of human high-molecular-mass kininogen. The amino acid sequence of the entire heavy chain and evidence for its evolution by gene triplication.
PubMed ID: 3828072
Title: Human Ile-Ser-bradykinin, identical with rat T-kinin, is a major permeability factor in ovarian carcinoma ascites.
PubMed ID: 2076202
Title: Ile-Ser-bradykinin is an aberrant permeability factor in various human malignant effusions.
PubMed ID: 4054110
Title: The amino acid sequence of the light chain of human high-molecular-mass kininogen.
PubMed ID: 3366244
Title: Isolation and identification of hydroxyproline analogues of bradykinin in human urine.
PubMed ID: 4952632
Title: Structural features of plasma kinins and kininogens.
PubMed ID: 7589467
Title: Purification from human plasma of a tetrapeptide that potentiates insulin-like growth factor-I activity in chick embryo cartilage.
PubMed ID: 6055465
Title: Second kininase in human blood plasma.
PubMed ID: 4322742
Title: A dipeptidyl carboxypeptidase that converts angiotensin I and inactivates bradykinin.
PubMed ID: 2989294
Title: Structural organization of the human kininogen gene and a model for its evolution.
PubMed ID: 3182782
Title: Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid from a patient with gastric cancer.
PubMed ID: 8760820
Title: Streptococcal cysteine proteinase releases kinins: a virulence mechanism.
PubMed ID: 12754519
Title: Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.
PubMed ID: 14760718
Title: Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.
PubMed ID: 16335952
Title: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.
PubMed ID: 19824718
Title: An initial characterization of the serum phosphoproteome.
PubMed ID: 19159218
Title: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.
PubMed ID: 19139490
Title: A strategy for precise and large scale identification of core fucosylated glycoproteins.
PubMed ID: 19838169
Title: Enrichment of glycopeptides for glycan structure and attachment site identification.
PubMed ID: 24275569
Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
PubMed ID: 26091039
Title: A Single Kinase Generates the Majority of the Secreted Phosphoproteome.
PubMed ID: 31087670#
Title: Hereditary angioedema